Human T cell enrichment for mLs not microliters
Updated on Sep 27, 2024 Share
Simplest Separation Ever: Human T Cell Enrichment for mLs, not microliters
Are magnetic columns and outdated processing techniques getting in the way of your workflow?
You’re not alone – processing a single sample can often require the use of multiple columns, the need to aliquot the sample volume into several smaller tubes, and lead to common headaches like clogged columns, inconsistent results between aliquots, and the need to babysit your samples… just to wind up with too few of your precious cells at the end.
Stop Losing Your Precious Samples to Magnetic Processes!
Sample preparation is a critical step to the success of downstream applications, but achieving high-quality results requires that delicate immune cells of interest be pure and unaltered by harsh retrieval techniques. Development of a gentle and effective method to obtain a high purity of a small target cell population from a larger volume sample is of the utmost importance.
Introducing Next-Gen Cell Separation with Akadeum’s Microbubbles
Akadeum’s Human T Cell Isolation Kits
Akadeum’s Human T Cell Isolation Kits allow for quick, gentle, and easy enrichment prior to cell sorting that is specifically designed to maintain the health and physiology of delicate cells of interest while maximizing retention, delivering a highly enriched population of target cells to enable better science. With Akadeum’s microbubbles, you can quickly and easily interrogate the full sample volume – directly in the sample container – using a fast and easy workflow.
|
Human T Cell Isolation Kit
Akadeum’s Human T Cell Isolation Kit leverages Akadeum’s novel Buoyancy Activated Cell Sorting (BACS™) technology for sample preparation that delivers a highly enriched population of healthy, viable T cells from Human Peripheral Blood Mononuclear Cells (PBMCs). |
|
Human CD4+ T Cell Isolation Kit
Akadeum’s Human CD4+ T Cell Isolation Kit delivers a highly-enriched population of healthy, viable CD4+ T cells from Human Peripheral Blood Mononuclear Cells (PBMCs) using our fast, easy, and gentle microbubble protocol that retains precious cells during the enrichment process, helping to maximize yield. |
No Magnet. No Column. Happy Cells.
Akadeum’s microbubble technology harnesses the power of buoyancy in a fast, easy, and gentle workflow that can interrogate the full sample volume directly in the sample container without the need for additional equipment. This unique, gentle approach delivers a highly-enriched population of happy, healthy T cells while maintaining cell health and physiology.
Elegant in Simplicity, Powerful in Application.
Akadeum’s innovative, buoyant approach to separation takes technology that traditionally requires small aliquots and additional equipment to accomplish this targeted cell separation and put it into a single container for self-separation. No harsh shear forces are applied, and precious samples are not exposed to external forces like rare earth magnets and magnetic gradients. Our exceptionally gentle approach to separation eliminates many of the existing technical hurdles that are limiting improvements or effectiveness of processes today.
The microbubbles are simply mixed into the sample where they seek out and bind to the unwanted contaminating cells, targeting them for removal. Using their inherent buoyancy, the microbubbles and their bound targets gently float to the surface of the sample container for removal, leaving the untouched cells of interest happy, healthy, and ready for downstream use.
See Akadeum’s Human T Cell Isolation Kits in Action
Akadeum’s Human T Cell Isolation Kits are being used in laboratories across the country to enable groundbreaking research and novel workflows across disciplines and institutions. Explore these resources to learn more:
Researcher Spotlight: Harvard University
Using Akadeum’s CD4+ T Cell Isolation to Study T-Cell Immune Responses in the Pathogenesis of Human Hepatotropic Viruses
>> Read the Researcher Spotlight
Akadeum’s Microbubble Approach to Cell Separation
Akadeum’s Buoyancy-Activated Cell Sorting (BACS™) microbubble technology represents a breakthrough in cell separation. The core of each microbubble is a hollow glass microsphere which allows researchers to leverage the power of gravity for quick and gentle removal of captured cells from a complex biological mixture.
With gravity as the driving force powering microbubble-based separation, Akadeum’s microbubble kits provide consistent results and eliminate the need for extraneous equipment and consumables like magnets and columns, while enabling reliable separations regardless of sample volume or container shape or size. Akadeum has taken technology that traditionally requires various equipment and consumables to perform and put it into a single container for self-separation that is exceptionally gentle on delicate cells.
Shop Akadeum’s Human T Cell Isolation Kits:
|
|
Human T Cell Isolation Kit
Akadeum’s Human T Cell Isolation Kit leverages Akadeum’s novel Buoyancy Activated Cell Sorting (BACS™) technology for sample preparation that delivers a highly enriched population of healthy, viable T cells from Human Peripheral Blood Mononuclear Cells (PBMCs). |
|
|
Human CD4+ T Cell Isolation Kit
Akadeum’s Human CD4+ T Cell Isolation Kit delivers a highly-enriched population of healthy, viable CD4+ T cells from Human Peripheral Blood Mononuclear Cells (PBMCs) using our fast, easy, and gentle microbubble protocol that retains precious cells during the enrichment process, helping to maximize yield. |
Let’s Start a Conversation!
If you are involved in cell isolation research, we’d love to hear from you! We are actively seeking new applications for our microbubble technology and would welcome the opportunity to learn more about your research to discover if our microbubbles could help you overcome longstanding hurdles in sample preparation. Our team of scientific experts is dedicated to the success of the researchers we work with, and we are looking forward to equipping you with our novel microbubble technology as another tool at your disposal as you work toward achieving the next big scientific breakthrough.