Applications

Published on Sep 30, 2024 Share

Akadeum’s Human T Cell Leukopak Isolation Kit was more effective at isolating cells than magnetic methods in ElevateBio’s CAR-T workflow. Read the app note to see how ElevateBio achieved high-purity T cells while reducing key cellular impurities. This led to improvements in overall cell therapy workflow efficacies and efficiencies. Key Data: Higher purity than magnetics and Improved removal of undesired cells.

Updated on Oct 3, 2024 Share

View performance data in this app note for the Cocoon® Platform while using Buoyancy-Activated Cell Sorting™ for T cell isolation, directly from cryopreserved leukopaks, with: No additional upstream equipment required, reduced operator interactions, flexibility of activator enabled by negative selection enables, recovery of ~64% of CD3+ cells with ~92% purity, and expansion to ~2.6×109 T cells in 9 days.

Updated on Sep 30, 2024 Share

Enhanced purity and yield of CD3+ T cells was achieved using Akadeum’s Human T Cell Leukopak Isolation Kit and sample material from a donor diagnosed with chronic lymphocytic leukemia (CLL) who has undergone multiple rounds of chemotherapy. Higher capture efficiency observed with Akadeum’s Human T Cell Selection, Activation, and Expansion Kit demonstrates a more effective recovery of viable T cells compared to magnetics.

Updated on Sep 30, 2024 Share

Isolation and activation of T cells with Akadeum’s Human T Cell Selection, Activation, and Expansion Kit can be complemented with expansion of the cells on the Wilson Wolf G-Rex® culture system. In this app note we demonstrate how the combination of the microbubbles and G-Rex® system promotes cell health and expansion while causing less cell exhaustion than magnetics.

Updated on Sep 30, 2024 Share

As your cell therapy workflow scales from research to commercialization, Akadeum’s technology is poised to scale with you. From small 50 million cell experiments to tens of billions of cells, performance and viability is maintained while time decreases.

Published on Sep 27, 2024 Share

Are you struggling with RBC contamination? Are lysis buffers damaging target cells or disturbing their normal physiology? Requiring only 3 simple steps and 10 minutes or less, Akadeum’s Human RBC Depletion Microbubbles remove contaminating red blood cells while keeping target cells happy and healthy. The entire process can be done directly in your sample container, no specialized equipment like magnets or columns necessary.

Published on Sep 27, 2024 Share

Dead cells are a common contaminant, and their presence can lead to any number of negative downstream impacts, including poor sort efficiencies, extended sort times, and an overall reduction in purity. Akadeum’s microbubble platform is uniquely well-suited for the targeted depletion of dead and dying cells while maintaining the health and physiology of live cells and other delicate analytes of interest.

Updated on Sep 27, 2024 Share

Processing Leukopaks for T Cells What Is a Leukopak? A leukopak is an enriched apheresis product collected via leukapheresis and made up of white blood cells, plasma, platelets, and red blood cells. Leukapheresis is a specialized application of apheresis protocol, which is designed to extract leukocytes—white blood cells—and return the remaining cells and plasma to the donor. The product of this procedure is a highly concentrated solution of blood components …

Updated on Sep 27, 2024 Share

Simplest Separation Ever: Human T Cell Enrichment for mLs, not microliters Are magnetic columns and outdated processing techniques getting in the way of your workflow? You’re not alone – processing a single sample can often require the use of multiple columns, the need to aliquot the sample volume into several smaller tubes, and lead to common headaches like clogged columns, inconsistent results between aliquots, and the need to babysit your …

Updated on Sep 27, 2024 Share

Sample preparation is critical to the success of downstream applications, but achieving high-quality results requires that delicate cells of interest be pure and unaltered by harsh retrieval techniques. There is a strong need for efficient, rapid, and gentle sample preparation methods to keep up with the advancements in technology for assessing numerous aspects of cellular and molecular biology.