Microbubbles for Cell Sorting and Sample Purification

Welcome to our blog. We are excited about microbubbles and using them for cell sorting. In this blog, we will discuss:

  • The features of microbubbles that make them a powerful tool in sorting cell and other biological targets
  • The background and basis of buoyancy-activated cell sorting (BACS™)
  • Various micrbubble applications, ranging from red blood cell removal to food pathogen preconentration (yes microbubbles can be used for both)
  • Technical perspectives from physics to biology

At Akadeum we care deeply about making meaningful products and having conversations with our customers. With this in mind, email us with any topic request, questions, or even if you are interested in a guest post.


Microbubbles Float Through Their First Flow Cytometry Conference

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As we discussed in a previous post, Akadeum recently attended the GLIIFCA 2016 meeting. John Younger, our CTO, gave a great talk to introduce Akadeum’s cell sorting technology, which generated some fantastic conversations. In the following days, we had a lot of attendees coming up to our booth asking, “So what are these microbubbles I heard about?”. Questions About Microbubbles and How They Are Used for Cell Sorting Probably the most common question though was about the material that microbubbles are made of and how they are used for cell sorting. If you are wondering as well, they are actually made of a glass shell filled with gas. John once quite aptly described them to me as the world’s smallest Christmas ornaments. So these Christmas-ornament-like hollow glass spheres are coated with antibodies, allowing the bubbles to specifically—and with high affinity—bind to target cells. As these microbubble-cell complexes float to the surface of the sample, the cells...

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Akadeum’s Cell Sorting Microbubbles to Appear at GLIIFCA 2016

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GLIIFCA 2016 and Akadeum The Akadeum Team is excited to present and attend this year’s Great Lakes International Imaging & Flow Cytometry Association (GLIIFCA) annual meeting. The meeting is held in Troy, MI this weekend on September 16-18th.The meeting provides a venue for flow cytometry researchers, cell separation researchers, and industry professionals to meet and share the latest updates on cell separation technologies. Started in 1992, this year will be the 25th annual meeting, and we couldn’t be more thrilled to be a part of it. Akadeum CTO to Present on Friday Night Our Chief Technical Officer and cofounder, John Younger, will be presenting at the Industry Science Symposium on September 16th. He will be sharing our game-changing technology with the Great Lakes flow cytometry community. As Akadeum’s Product Manager, I’m also looking forward to getting feedback and ideas about microbubble cell sorting technology from experts in the field. Whenever we get a chance,...

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Can microbubbles be pelleted by centrifugation?

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In short, no. At least not a pellet that you would see at the bottom of your tube when you spin down cells. Instead, what happens is anything with a density greater than water sinks and sinks faster when in a centrifuge. And anything with a density less than water rises and rises faster when in a centrifuge. So, microbubbles all go to the same place in a tube, but instead of being at the bottom they are at the top. This is also true for any cells that are attached to the microbubbles, they float to the top when centrifuged. While at an entirely different scale, another way to think about this is from the time you might have spent in a pool in your childhood. Granted, what a cell on a microbubble experiences is at a much lower Reynolds number, it can still serve as a useful analogy. If you were to strap...

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