Enhancing T Cell Isolation with Akadeum’s Alerion™ Microbubble Cell Separation System: A Case Study With Comprehensive Cell Solutions  

Updated on Nov 22, 2024 Share

Comprehensive Cell Solutions (CCS), a business unit of the New York Blood Center Enterprises, is a Contract Development and Manufacturing Organization. CCS recently evaluated the Alerion™ Microbubble Cell Separation System, the BioRise™ consumable, and the negative selection Human T Cell Leukopak Isolation Kit. CCS is dedicated to streamlining their manufacturing process and requires a platform that is flexible, efficient, and consistently delivers high-performance results to meet the demands of cell therapy manufacturing and development.

Kit Used

The Alerion™ Microbubble Cell Separation System was paired with Akadeum’s Human T Cell Leukopak Isolation Kit and BioRise™ consumable.

Experimental Design

To conduct this evaluation, CCS performed a split-run experiment with apheresis material from two healthy donors. Each leukopak was washed following CCS’s standard washing procedure and then split in half, approximately 7 billion cells in each half. Each half from both donors was then processed using the Alerion™ Microbubble Cell Separation System and the BioRise™ consumable in combination with Akadeum’s negative selection Human T Cell Leukopak Isolation Kit or a standard positive selection magnetic-based kit and corresponding instrument.

Figure 1. Superior impurity depletion using microbubbles.

Figure 2. Untouched T cells isolated via the Alerion™ Microbubble Cell Separation System result in greater post-thaw viability compared to magnetics-based methods.

Discussion

The Alerion™ Microbubble Cell Separation System and the BioRise™ consumable, combined with Akadeum’s negative selection Human T Cell Leukopak Isolation Kit, provide unmatched results. 

• Enhanced impurity depletion: Akadeum’s platform depletes B cells and monocytes, two cell types that can hinder T cell expansion and transduction, more effectively compared to the conventional magnetic platform.
• Greater viability: The Alerion™ Microbubble Cell Separation System isolated samples maintained over 12% greater viability for each donor after cryopreservation than the conventional magnetic platform. When developing cell therapies, preserving cell health throughout processing is critical for efficacy. 

Conclusion

Akadeum’s Alerion™ Microbubble Cell Separation System, the BioRise™ consumable, and the negative selection Human T Cell Leukopak Isolation Kit provided CCS with enhanced results compared to the conventional magnetic method. The Alerion™ was able to process faster and provide more pure T cell populations while also maintaining cell health. Ultimately, producing a higher-quality intermediate, giving the potential for a better-quality product downstream.

Ready to learn more about how the Alerion™ Microbubble Cell Separation System can improve your T cell banking and cell therapy workflow? Contact our team today to schedule a demo or purchase a Human T Cell Leukopak Isolation Kit.