Enhancing T Cell Isolation with Akadeum’s Alerion™ Microbubble Cell Separation System: A Case Study With Comprehensive Cell Solutions  

Updated on Oct 5, 2024 Share

Comprehensive Cell Solutions (CCS), a business unit of the New York Blood Center Enterprises, is a Contract Development and Manufacturing Organization. CCS recently evaluated the Alerion™ Microbubble Cell Separation System, the BioRise™ consumable, and the negative selection Human T Cell Leukopak Isolation Kit. CCS is dedicated to streamlining its manufacturing process and requires a platform that is flexible, efficient, and consistently delivers high-performance results to meet the demands of cell therapy manufacturing and development.

Kit Used

The Alerion™ Microbubble Cell Separation System was paired with Akadeum’s Human T Cell Leukopak Isolation Kit and BioRise™ consumable.

Experimental Design

To conduct this evaluation, CCS performed a split-run experiment with apheresis material from two healthy donors. Each leukopak was washed following CCS’s standard washing procedure and then split in half, approximately 7 billion cells in each half. Each half from both donors was then processed using the Alerion™ Microbubble Cell Separation System and the BioRise™ consumable in combination with Akadeum’s negative selection Human T Cell Leukopak Isolation Kit or a standard positive selection magnetic-based kit and corresponding instrument.

Figure 1: Superior impurity depletion using microbubbles.

Figure 2: Alerion™ microbubble-based cell isolation results in more T cells for cell therapy.

Discussion

The Alerion™ Microbubble Cell Separation System and the BioRise™ consumable, combined with Akadeum’s negative selection Human T Cell Leukopak Isolation Kit provide unmatched results:

• Enhanced impurity depletion: Akadeum’s platform depletes B cells and monocytes, two cell types that can hinder T cell expansion and transduction, more effectively compared to the conventional magnetic platform.
• Greater T cell recovery: The Alerion™ Microbubble Cell Separation System recovered over 45% more T cells for each donor than the conventional magnetic platform. When developing cell therapies, preserving a greater number of T cells from the apheresis material not only aids in efficiency but also reduces overall costs.

Conclusion

Akadeum’s Alerion™ Microbubble Cell Separation System, the BioRise™ consumable, and the negative selection Human T Cell Leukopak Isolation Kit provided CCS with enhanced results compared to the conventional magnetic method. The Alerion™ was able to process faster and provide more pure T cell populations while also recovering a greater number of T cells. Ultimately producing a higher-quality intermediate, resulting in a superior-quality product downstream.

Ready to learn more about how the Alerion™ Microbubble Cell Separation System can improve your T cell banking and cell therapy workflow? Contact our team today to schedule a demo or purchase a Human T Cell Leukopak Isolation Kit.