FAQs

BACS is Akadeum’s proprietary cell separation technology that uses buoyant microbubbles to isolate specific cell populations utilizing only the power of gravity.

Akadeum’s microbubbles are hollow silica spheres that are on average 12 microns.  

Microbubbles average 12 um in diameter. Learn more about microbubbles technology. 

Microbubbles can carry as many as four cells.  

Ca2+, Mg2+ free Dulbecco’s PBS containing 2mM EDTA, and 0.5% bovine serum albumin (BSA). BSA must be biotin free to not interfere with kit performance. 

No, this is normal due to the buoyant properties of microbubbles. The solution will need to be mixed well before every use. If processing multiple tubes, mixing between tubes is necessary.  

Aspiration is the best and easiest way to remove the microbubble layer after centrifugation. Pipetting off the bubble layer is not very effective. Vacuum pumps are available from a variety of vendors. 

Kits are shipped on ice packs with overnight delivery. 

It is not recommended to process under 10 million cells per sample. The maximum number of cells processed is dependent on the size of the vessel as each kit is very scalable.  

Akadeum’s Human T Cell Leukopak Isolation Kit is the first ever negative selection kit that can meet the scale requirements for cell therapy. The Human T Cell Depletion Kit also uses negative selection to deplete endogenous CD3+ T cells. The Human T Cell Selection, Activation, and Expansion Kit uses positive selection.  

Washed leukopak material refers to a leukopak that has been processed to reduce platelets, exchange buffer, and concentrate the cells. This can be done manually via centrifugation or by using commercially available cell washing instruments.  

Yes, Akadeum has successfully processed raw leukopaks. Please email techsupport@akadeum.com to learn more.  

Yes, Akadeum’s kits are designed to work with both fresh and cryopreserved samples. 

Akadeum’s kits can be integrated into both autologous and allogeneic workflows. 

45 minutes 

The number of cells processed depends on the specific kit or customized protocol. In general, each BioRise™ consumable can process 3 – 50 billion cells each. The Alerion™ is capable of processing two consumables simultaneously.  

Two leukopaks can be processed simultaneously, each in their BioRise™ consumable which provides a closed system. Processing two consumables at a time allows for one technician to easily process 10 leukopaks in a single shift.  

Yes, reach out to your Account Executive or email info@akadeum.com to learn more! 

Yes. This can be specifically helpful when using the Dead Cell Removal Microbubble Kit or the Human Red Blood Cell Depletion Microbubbles.  

Akadeum’s Dead Cell Removal Microbubble Kit utilizes Annexin V-coated microbubbles to selectively bind phosphatidylserine, a marker exposed on the surface of cells undergoing apoptosis. This technology enables efficient removal of apoptotic cells across various cell types where apoptosis is actively occurring.  

Although the initial cell viability is below the recommended threshold, successful outcomes have been achieved by performing the protocol twice on such samples.