Akadeum’s Human T Cell Depletion Kit uses microbubbles to remove unedited CD3+ T cells from CAR T cultures. Unwanted T cells expressing CD3 are positively isolated using a cocktail of biotinylated anti-CD3 antibodies, which are then captured using our buoyant Human T Cell Depletion Microbubbles that gently float the targeted cells to the top of the sample. This leaves a highly pure sample of engineered cells untouched and ready for downstream formulation. The highly scalable nature of this process means that it can easily be incorporated into a wide variety of cell therapy manufacturing workflows.
Scalable to fit your cell therapy workflow needs.
Highly pure sample of CD3 negative cells post-depletion.
Increased recovery of gene-edited cells for downstream formulation.
Flexibility to integrate into many existing workflows.
Gentle process maintains the viability of the cell therapy product.
Available in two sizes: 1 Billion Cells and 5 Billion Cells.
Akadeum’s process offers an elegant solution for highly effective and scalable T cell depletion. Biotinylated anti-CD3 antibodies bind to untransduced T cells still expressing the CD3 epitope. Streptavidin coated Human T Cell Depletion Microbubbles then selectively bind to the antibodies labeling the undesired cells. Due to inherent buoyancy, the microbubbles naturally float to the surface of the culture vessel bringing along CD3+ cells. The bubble-bound cells can then be easily extracted off the surface with the depletion microbubbles, leaving untouched engineered cells.
Additional Information:
5 Billion cell kit is compatible with manual processing, in-bag workflows, and the Alerion™ Microbubble Cell Separation System
Storage: 4°C
Cell Type: T Cells
Sample Species and Source: Engineered Human CAR T cell cultures
Cell Separation Method: Positive (Depletion)
This Kit Comes With:
1 Billion Kit:
10 mL Human T Cell Depletion Microbubbles (in storage buffer)
1 mL Human T Cell Depletion Antibody Cocktail
5 Billion Kit:
50 mL Human T Cell Depletion Microbubbles (in storage buffer)
Go from research scale to commercialization with microbubbles. Just use more or less to suit your research needs and keep performance maintained.
*Only the 5 Billion Cell kit is compatible with the BioRise™
Experimental Overview:
PBMC input material was prepared from washed apheresis cells and additional RBC lysis. Depletion was completed in accordance with the Human T Cell Depletion Kit protocol; isolations were performed in a BioRise™Consumable using the Alerion™ Microbubble Cell Separation System. Averaged results from three unique donors comparing BioRise™ depletions (5B total cells) to small (50M total cells) and mid scale (1B total cells) performed manually in a 5mL tube or 150mL bag, respectively. Depletion was calculated based on remaining T cells in the negative fraction.
The Right Products to Support Your Pipeline:
The Human T Cell Depletion Kit is ideal for research through process development, with GMP and Clinical Ready versions available to support preclinical to commercialization of cell therapy workflows.
Data
Experimental Overview:
PBMC input material (5B total cells per donor) were prepared from washed apheresis material and additional RBC lysis. Depletion was completed in accordance with the Human T Cell Depletion Kit protocol; isolations were performed in a BioRise™ Consumable using the Alerion™ Microbubble Cell Separation System. The donor-specific CD3 depletion is shown with the average CD3 depletion (indicated by the dotted line). Depletion was calculated based on remaining T cells in the negative fraction.
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