Product Details
How it Works: Leukopak samples contain a milieu of immune cells (i.e., white blood cells), plasma, platelets, and red blood cells. The Human PBMC Leukopak Isolation Kit uses a chemical-free, gentle negative selection method to enrich leukopak PBMCs by removing unwanted cell populations.
These unwanted cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD66b, CD123, and CD235ab. BACS™ Streptavidin Microbubbles are mixed into the sample where they bind to the labeled cells. Due to their inherent buoyancy, the microbubbles quickly and gently float the non-PBMCs to the surface. Following centrifugation, the PBMCs are available for collection.
The Human PBMC Leukopak Isolation Kit minimizes handling steps and consistently delivers more isolated PBMCs. Resulting PBMCs from the Akadeum kit have the benefit of being untouched and not being exposed to chemical processes. Isolated leukopak PBMCs are suitable for flow cytometry, molecular assays, activation and expansion, cell culture, cell engineering or other functional studies.
Additional Information:
- Storage: 4°C
- Cell Type: PBMCs
- Sample Species and Source: washed human leukopak apheresis material
- Cell Separation Method: Negative Selection
- Capacity:
- Human PBMC Leukopak Isolation Kit: 12.5 x 109 total cells (enough to process an average half-leukopak)
- Human PBMC Leukopak Isolation Kit, Research Scale: 2.0 x 109 total cells
- Research Use Only
This Kit Comes With:
Human PBMC Leukopak Isolation Kit:
- 62.5 mL BACS™ Streptavidin Microbubbles
- 12.5 mL Human PBMC Leukopak Biotin-Antibody Cocktail
- 500 mL Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA)
Human PBMC Leukopak Isolation Kit, Research Scale:
- 10 mL BACS™ Streptavidin Microbubbles
- 1 mL Human PBMC Leukopak Biotin-Antibody Cocktail
- 200 mL Separation Buffer (Ca2+ and Mg2+ free PBS containing 2 mM EDTA, 0.5% biotin-free BSA)
