The Human T Cell Leukopak Isolation Kit was developed with BACS™ Microbubbles to isolate untouched T cells from washed leukopak material via negative selection. Leveraging the gentleness of flotation, non-T cells are targeted and removed with antibodies. Negatively selected T cells are suitable for a wide variety of downstream assays, including cell culture, flow cytometry, scRNA-Seq, engineering, or other functional studies. The Human T Cell Leukopak Isolation Kit has the capacity to isolate T cells from an average half-leukopak (12.5 x 10⁹ total cells).
Leukopak to purified cells in less than one hour, no matter the cell input number
Higher recovery of healthier T cells to enhance CAR-T research
Reduce cell impurities and improve your transduction efficiencies
Scale up to tens of billions of cells: compatible with the Alerion™ Microbubble Cell Separation System
Negative selection workflow eliminates concerns of particles directly bound to T cells
How it Works: The Human T Cell Leukopak Biotin Antibody Cocktail is added to the leukapheresis product to label unwanted cell populations. BACS™ Streptavidin Microbubbles are added to the sample and bind to the biotin-labeled cells. Due to their inherent buoyancy, the microbubbles quickly and gently float the non-T cells to the surface. Following centrifugation, the untouched T cells in their original state are collected.
Additional Information:
Compatible with the Alerion™ Microbubble Cell Separation System
Go from research scale to commercialization scale with microbubbles. Just use more or less to suit your research needs.
Example of scale: 50 million cells in a tube, to 1 billion cells in bag, to 18 billion cells in a BioRise™ Consumable.
Apheresis material was platelet washed using Thermo Fisher Scientific’s Rotea™ cell washing instrument and then isolated using Akadeum’s negative selection Human T Cell Leukopak Isolation Kit. Samples were processed either in a 50 mL tube at 500 million total starting cells or in a BioRise™ Consumable on the Alerion™ Microbubble Cell Separation System at 5-15 billion cells per consumable. These assays were performed in triplicate.
The Right Products to Support Your Pipeline
The Human T Cell Leukopak Isolation Kit is ideal for research through process development, but rest assured additional grades are and will be available for the remaining stages of the path to commercialization of cell therapy workflows.
Data
High Purity and Yield at Any Scale
Untouched T cells were isolated from platelet-washed leukopak material using the Human T Cell Leukopak Isolation Kit in accordance with the published protocol. Isolation was completed with either 100 million cells in a 5 mL tube or 1 billion cells in a 50 mL tube. Samples were run in triplicate. To determine T cell purity and yield, isolated T cells and an aliquot of the initial sample were labeled with CD45-FITC and CD3-APC Fire/750. The fluorescently labeled cells were analyzed by flow cytometry. Dead cells and debris were excluded from the analysis.
Yield and purity for a three-donor leukapheresis sample enriched for T cells with a baseline purity of 41.7% with either the negative selection Human T Cell Leukopak Isolation Kit or a magnetics-based positive selection workflow demonstrates superior performance of Akadeum’s Human T Cell Leukopak Isolation Kit. Data courtesy of third party CDMO.
Resources
Protocol Sheet
For processing on the Alerion™, please refer to the:
App Notes
Publications & References
Starting with RUO? See the GMP kit flyer for your next phase of kit needs.
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