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Learn About Alerion™ Microbubble Cell Separation System – Scalable. Fast. Efficient.
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Microbubble Demo
Share Category: BACSTM Microbubble Technology and Workflow
See our Microbubbles in Action! With nothing but a small shake or stir, microbubbles spring into action sorting target cells in a quick and gentle manner. Their small size allows this process to take place in virtually any container.
Microbubble Workflow
Share Category: BACSTM Microbubble Technology and Workflow
Microbubbles are Fast, Easy, and Gentle! Suspend a sample in up to 50M cells in 100 uL separation buffer Draw up microbubbles with a recommended ratio of 1 microbubble for each target cell Mix bubbles with sample, stir 30 strokes over 1 minute with a standard pipette tip Use a Pasteur pipette to aspirate target cells from the top of the solution Resuspend cells, preparing them for use in a …
Magnetic Separation
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Magnetic Separation Workflow Many biotech companies are still using magnetic separation, in this process the target cell is captured by a magnetic particle, and a magnetic force pulls the target particles to one side. The amount of force generated depends on the proximity to a rare earth magnet. The non-target cells are removed while the target cells are left behind. A new buffer is added to suspend the magnetic particles. …
Akadeum’s Nucleic Acid Extraction Automation
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Akadeum Life Sciences was founded with the purpose of advancing human health, and in these unprecedented times, we are actively working to contribute to the global scientific community and its understanding of COVID-19 as well as the development of advanced detection capabilities. Automating the microbubble-based nucleic acid extraction workflow To save time, improve throughput, and increase efficiency, it is critical that Akadeum’s nucleic acid extraction microbubble workflow can be automated …
Akadeum’s Negative Selection Workflow
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Akadeum’s Negative Selection cell enrichment kits use streptavidin-coated microbubbles and an optimized cocktail of biotinylated antibodies to label, capture, and remove unwanted cell populations. This workflow takes about half an hour from start to finish, and is exceptionally gentle on delicate immune cells. Using one of Akadeum’s kits for sample preparation can deliver a highly-enriched population of happy, healthy cells for downstream processing.
Introduction to Akadeum
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At Akadeum Life Sciences, we have developed a revolutionary approach to sample preparation that uses our proprietary, buoyant microbubble technology to quickly and easily enrich your sample for the analyte(s) of interest while maintaining target cell health and physiology. Elegant in Simplicity, Powerful in Application Our buoyant approach to separation eliminates many of the existing technical hurdles that are limiting improvements or effectiveness of processes today. Unlike with magnetic bead-based …
A Superior Dead Cell Removal Platform Using Microbubbles
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Catch the highlights from Akadeum’s webinar: A Superior Dead Cell Removal Platform Using Microbubbles. Dead cells are a common headache when it comes to sample preparation. Their presence can lead to any number of negative downstream impacts, including poor sort efficiencies, extended sort times, and an overall reduction in purity. Buoyancy-Activated Cell SortingTM (BACSTM), Akadeum’s patented approach to separation, offers a novel approach to Dead Cell Removal (DCR). Akadeum’s microbubble …
Working with Microbubbles: Aspiration Best Practices
Share Category: BACSTM Microbubble Technology and Workflow
Working with Microbubbles: Aspiration Best Practices For the most effective microbubble removal, vacuum aspiration is recommended. Here we are using a glass Pasteur pipette to aspirate the microbubble layer. Insert the pipette, bringing it down along the side wall. Aim for the top edge of the microbubble layer where it meets the side of the vial. Carefully vacuum away the microbubble layer, moving the pipette tip around the side wall …
Full Webinar: Reaching New Heights with Nucleic Acid Extraction
Saliva presents an excellent option for non-invasive, self-collected samples for diagnostic testing. Research has established that saliva can be used to quantifiably measure analytes in a way that is predictive of disease presentation, though technical hurdles have prevented efficient and cost-effective scaling that would allow widespread adoption of saliva testing. Realization of the full potential of saliva testing has been impeded by the inherent limitation of magnetic technologies, especially with …